Difference FT-IR for examination of comformational change of bovine heart cytochrome c oxidase.
نویسندگان
چکیده
منابع مشابه
IR signatures of the metal centres of bovine cytochrome c oxidase: assignments and redox-linkage.
Assignments of IR bands of reduced minus oxidized IR difference spectra of bovine and related cytochrome c oxidases are reviewed and their linkages to specific metal centres are assessed. To aid this, redox-poised difference spectra in the presence of cyanide or carbon monoxide are presented. These ligands fix the redox states of either haem a3 alone or haem a3 and CuB respectively, while allow...
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As I mentioned at the awards ceremony a little while ago, the work for which I have received the Keio Med ical Award is not something that I have been able to accomplish alone; it has involved the cooperation of many scientists. Dr. Tsukihara is Professor of the Insti tute for Protein Research, Osaka University, and spe cializes in X-ray crystallography. Dr. Kitagawa and Dr. Ogura, who is here ...
متن کاملStructures and physiological roles of 13 integral lipids of bovine heart cytochrome c oxidase.
All 13 lipids, including two cardiolipins, one phosphatidylcholine, three phosphatidylethanolamines, four phosphatidylglycerols and three triglycerides, were identified in a crystalline bovine heart cytochrome c oxidase (CcO) preparation. The chain lengths and unsaturated bond positions of the fatty acid moieties determined by mass spectrometry suggest that each lipid head group identifies its ...
متن کاملQuantitative reevaluation of the redox active sites of crystalline bovine heart cytochrome c oxidase.
Approximately 30% of the iron contained in a bovine heart cytochrome c oxidase preparation was removed by crystallization, giving a molecular extinction coefficient 1.25-1.4 times higher than those reported thus far. Six electron equivalents provided by dithionite were required for complete reduction of the crystalline cytochrome c oxidase preparation. The fully reduced enzyme was oxidized with...
متن کاملCrystalline cytochrome c oxidase of bovine heart mitochondrial membrane: composition and x-ray diffraction studies.
The integral mitochondrial membrane protein cytochrome c oxidase (ferrocytochrome-c:oxygen oxidoreductase, EC 1.9.3.1) was crystallized from solutions of the protein from bovine heart isolated as described earlier [Yoshikawa, S. & Caughey, W. S. (1982) J. Biol. Chem. 257, 412-420]. Crystallinity was demonstrated by x-ray diffraction. Microcrystals (tetragonal prisms, 0.02 mm in the largest dime...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2001
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.41.s114_3